Student projects ('stage')

Students can participate in a any of a number of currentprojects, including functional and mechanistic studies of transcriptional regulation and promoters, epigenetic regulation of early development.

Techniques used in the lab

- State-of-the-art techniques such as massive parallel sequencing of cDNA (RNA-seq) for transcriptome profiling, or deep sequencing of DNA obtained by chromatin immunoprecipitation (ChIP-seq) to analyze in vivo binding in the genome

- Bioinformatics approaches for the analysis of large genomic data sets

- Molecular Biology (subcloning, RNA and DNA isolation, PCR, Northern and Southern blotting, site-directed mutagenesis, RNA synthesis, antisense knockdown technology)

- Biochemistry (protein purification, SDS-PAGE, Western blotting, immunoprecipitation)

- Animal experimental techniques (embryo micro-injection, experimental embryology, microscopy)

Links to disease

- Research of embryonic gene regulation is important to understand pluripotency and to use stem cells in regenerative medicine

- Research of embryonic gene regulation is important to increase our understanding of congenital disease and of deregulation of growth and differentiation, such as in cancer.

- Elongation of the CAG repeat coding for poly glutamine in TBP causes dominant spino-cerebellar ataxia (SCA 17) and a Huntington's Disease-like phenotype.

- Mutations in methylated DNA-binding protein MeCP2 cause Rett Syndrome, an X-linked mental retardation syndrome.

More information

For more information please contact us by email, or visit one of the following links:
- Research topics (general information)
- Advantages of using Xenopus as a model system
- Background: Gene expression during development

Radboud University Faculty of Science Radboud Institute for Molecular Life Sciences Dept. Molecular Developmental Biology