Student projects ('stage')
Students can participate in a any of a number of currentprojects, including functional and mechanistic studies of transcriptional regulation and promoters, epigenetic regulation of early development.
Techniques used in the lab
State-of-the-art techniques such as massive parallel sequencing of cDNA (RNA-seq) for transcriptome profiling, or deep sequencing of DNA obtained by chromatin immunoprecipitation (ChIP-seq) to analyze in vivo binding in the genome
Bioinformatics approaches for the analysis of large genomic data sets
Molecular Biology (subcloning, RNA and DNA isolation, PCR, Northern and Southern blotting, site-directed mutagenesis, RNA synthesis, antisense knockdown technology)
Biochemistry (protein purification, SDS-PAGE, Western blotting, immunoprecipitation)
Animal experimental techniques (embryo micro-injection, experimental embryology, microscopy)
Links to disease
Research of embryonic gene regulation is important to understand pluripotency and to use stem cells in regenerative medicine
Research of embryonic gene regulation is important to increase our understanding of congenital disease and of deregulation of growth and differentiation, such as in cancer.
Elongation of the CAG repeat coding for poly glutamine in TBP causes dominant spino-cerebellar ataxia (SCA 17) and a Huntington's Disease-like phenotype.
Mutations in methylated DNA-binding protein MeCP2 cause Rett Syndrome, an X-linked mental retardation syndrome.
More informationFor more information please contact us by email, or visit one of the following links:
Research topics (general information)
Advantages of using Xenopus as a model system
Background: Gene expression during development