Core promoters elements in Xenopus tropicalis

 

Transcription start sites

A collection of transcription start sites (TSSs) was obtained by TSS-seq (a CAGE-related approach adapted for deep sequencing) of oocyte and gastrula (stage 10-12) RNA. The TSSs were compared to likely promoter locations using ChIP-sequencing of the general transcription initiation factor TBP and histone H3 K4 trimethylation. The TSSs were used to predict Xenopus core promoter elements, and compare the abundance of these sequence elements in Xenopus and human promoters.

TSS-seq tracks


Publications

Van Heeringen, S.J., W. Akhtar, U.G. Jacobi, R.C. Akkers, Y. Suzuki, G.J.C. Veenstra. 2011. Nucleotide composition-linked divergence of vertebrate core promoter architecture. Genome Research, 21, 410-421.
www Genome Research Online          Email

Downloads

Visualization and annotation tracks can be used as custom tracks in the UCSC Genome Browser using the links below. These UCSC visualization tracks represent summed read counts at 1bp or 10 bp resolution for TSS-seq and ChIP-seq tracks respectively (available in wiggle format). The Excel table contains the filtered TSS genomic coordinates and the FASTA file (GZip compressed) contains the corresponding promoter sequences from -400 to +100 relative to the TSS.

Assembly: Joint Genome Institute version 4.1 (xenTro2, August 2005).

File Name: Date Created: File Size:
 Supplemental_Table_S1_replaced.xls  9/7/2012 299 KB
 TBP.wig.gz  9/7/2012 15,719 KB
 TSS-seq_tracks.wig.gz  9/7/2012 554 KB
 TSS_-400+99_norepeatmask.fasta.gz  9/7/2012 575 KB


The raw data have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE21482

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Radboud University Faculty of Science Radboud Institute for Molecular Life Sciences Dept. Molecular Developmental Biology